293t american type culture collection cells Search Results


293 t cell line  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection 293 t cell line
HBx and TGF-β1 exposure induce upregulation of miR-199a-3p through JNK/c-Jun pathway. a Comparison of miR-199a-3p expression in the indicated cells. b Left panel, HBx expression was confirmed by way of qRT-PCR in LE/6 cells treated with pcDNA3.1-HBx. Right panel, c-Jun and p-c-Jun expression were analyzed by western blot in LE/6 cells treated with pcDNA3.1-HBx. c LE/6 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). d <t>Huh7</t> cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). n = 3 per group, data represent mean ± SEM, P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001
293 T Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293 t cell line - by Bioz Stars, 2026-02
90/100 stars
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293 t cells  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection 293 t cells
HBx and TGF-β1 exposure induce upregulation of miR-199a-3p through JNK/c-Jun pathway. a Comparison of miR-199a-3p expression in the indicated cells. b Left panel, HBx expression was confirmed by way of qRT-PCR in LE/6 cells treated with pcDNA3.1-HBx. Right panel, c-Jun and p-c-Jun expression were analyzed by western blot in LE/6 cells treated with pcDNA3.1-HBx. c LE/6 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). d <t>Huh7</t> cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). n = 3 per group, data represent mean ± SEM, P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001
293 T Cells, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/293 t cells/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
293 t cells - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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HBx and TGF-β1 exposure induce upregulation of miR-199a-3p through JNK/c-Jun pathway. a Comparison of miR-199a-3p expression in the indicated cells. b Left panel, HBx expression was confirmed by way of qRT-PCR in LE/6 cells treated with pcDNA3.1-HBx. Right panel, c-Jun and p-c-Jun expression were analyzed by western blot in LE/6 cells treated with pcDNA3.1-HBx. c LE/6 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). d Huh7 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). n = 3 per group, data represent mean ± SEM, P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Oncogene

Article Title: TGF-β1 accelerates the hepatitis B virus X-induced malignant transformation of hepatic progenitor cells by upregulating miR-199a-3p

doi: 10.1038/s41388-019-1107-9

Figure Lengend Snippet: HBx and TGF-β1 exposure induce upregulation of miR-199a-3p through JNK/c-Jun pathway. a Comparison of miR-199a-3p expression in the indicated cells. b Left panel, HBx expression was confirmed by way of qRT-PCR in LE/6 cells treated with pcDNA3.1-HBx. Right panel, c-Jun and p-c-Jun expression were analyzed by western blot in LE/6 cells treated with pcDNA3.1-HBx. c LE/6 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). d Huh7 cells transiently transfected with pcDNA3.1-vec or pcDNA3.1-HBx were treated with/ without TGF-β1 or sp600125 for 24 h. The expression of miR-199a-3p were examined by qRT-PCR (upper) and the expression of HBx, c-Jun, p-c-Jun, JNK, p-JNK were determined by western blot (lower). n = 3 per group, data represent mean ± SEM, P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: 293 T and Huh7 cell lines were purchased from the China Center for Type Culture Collection (Wuhan, China), and maintained in DMEM (Gibco, USA) medium.

Techniques: Comparison, Expressing, Quantitative RT-PCR, Western Blot, Transfection

HBx and TGF-β1 activate miR-199a-3p promoter through transcriptional factor c-Jun. a Relative luciferase activities of pGL4.17 and pGL4.17-2000 were measured in Huh7 cells transfected with pcDNA3.1 vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). b Schematic of miR-199a-3p promoter constructs. Relative luciferase activities of pGL4.17 containing various length of miR-199a-3p promoter regions were determined in Huh7 cells exposed to HBx (upper) and TGF-β1(lower). c Huh7 cells were cotransfected with pGL4.17-482 and pcDNA3.1-HBx at different doses, and luciferase activities were analyzed. Huh7 cells were transfected with pGL4.17-482 and then treated with TGF-β1 at different doses, and luciferase activities were analyzed. d Luciferase activities of wild-type (pGL4.17-482) or c-Jun response element mutant (pGL4.17-482-mut) miR-199a-3p promoter were examined in Huh7 cells cotransfected with control vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). e Huh7 cells were transiently transfected with control vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). The cell lysates were subjected to chromatin immunoprecipitation (ChIP) analysis with immunoglobin G or anti-c-Jun antibody. Pull-down DNA was analyzed by qRT-PCR using specific primer targeting the c-Jun binding site in miR-199a-3p promoter. n = 3 per group, data represent mean ± SD ( a–d ) or mean ± SEM ( e ), P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Oncogene

Article Title: TGF-β1 accelerates the hepatitis B virus X-induced malignant transformation of hepatic progenitor cells by upregulating miR-199a-3p

doi: 10.1038/s41388-019-1107-9

Figure Lengend Snippet: HBx and TGF-β1 activate miR-199a-3p promoter through transcriptional factor c-Jun. a Relative luciferase activities of pGL4.17 and pGL4.17-2000 were measured in Huh7 cells transfected with pcDNA3.1 vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). b Schematic of miR-199a-3p promoter constructs. Relative luciferase activities of pGL4.17 containing various length of miR-199a-3p promoter regions were determined in Huh7 cells exposed to HBx (upper) and TGF-β1(lower). c Huh7 cells were cotransfected with pGL4.17-482 and pcDNA3.1-HBx at different doses, and luciferase activities were analyzed. Huh7 cells were transfected with pGL4.17-482 and then treated with TGF-β1 at different doses, and luciferase activities were analyzed. d Luciferase activities of wild-type (pGL4.17-482) or c-Jun response element mutant (pGL4.17-482-mut) miR-199a-3p promoter were examined in Huh7 cells cotransfected with control vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). e Huh7 cells were transiently transfected with control vector or pcDNA3.1-HBx (left) and treated with or without TGF-β1 (right). The cell lysates were subjected to chromatin immunoprecipitation (ChIP) analysis with immunoglobin G or anti-c-Jun antibody. Pull-down DNA was analyzed by qRT-PCR using specific primer targeting the c-Jun binding site in miR-199a-3p promoter. n = 3 per group, data represent mean ± SD ( a–d ) or mean ± SEM ( e ), P values were calculated by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: 293 T and Huh7 cell lines were purchased from the China Center for Type Culture Collection (Wuhan, China), and maintained in DMEM (Gibco, USA) medium.

Techniques: Luciferase, Transfection, Plasmid Preparation, Construct, Mutagenesis, Control, Chromatin Immunoprecipitation, Quantitative RT-PCR, Binding Assay